The Rat Adiponectin ELISA Kit is specifically designed for the quantitative detection of Adiponectin (also known as ADP or Acrp30) levels in various rat biological samples. Adiponectin is a vital adipokine that plays a significant role in metabolic processes, including insulin sensitivity, glucose regulation, and lipid metabolism. As a key regulator of energy homeostasis, Adiponectin is involved in modulating various cellular functions related to obesity, diabetes, cardiovascular diseases, and inflammation. Accurate measurement of Adiponectin levels using this ELISA kit provides researchers with valuable insights into the intricate mechanisms underlying metabolic regulation and its impact on physiological pathways. By quantifying Adiponectin with exceptional sensitivity and specificity, this kit enables detailed investigations into the role of Adiponectin in metabolic disorders, insulin resistance, and obesity-related complications. The Rat Adiponectin ELISA Kit offers reliable and reproducible results, allowing researchers to study the intricate relationship between Adiponectin levels and metabolic diseases. Manufactured under stringent quality control standards, this kit ensures robust performance and user-friendly protocols, making it an excellent choice for research studies focusing on adipokine biology, metabolic disorders, and related therapeutic strategies.
Product Name:
Rat ADP/Acrp30 (Adiponectin) ELISA Kit
SKU:
AEES00528
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
