Description
Rat ANP (Atrial Natriuretic Peptide) ELISA Kit
The Rat ANP (Atrial Natriuretic Peptide) ELISA Kit is precisely tailored to enable the quantitative determination of Atrial Natriuretic Peptide (ANP) levels in a variety of rat biological samples. ANP, a vital hormone prominently secreted from the heart, plays a key role in regulating blood pressure, fluid balance, and electrolyte homeostasis. Its involvement in cardiovascular physiology and pathophysiology underscores the importance of accurate ANP measurement in comprehending cardiovascular health, cardiovascular diseases, and renal function.
With a focus on sensitivity and specificity, Assay Genie's ANP ELISA Kit ensures accurate and reproducible results, making it the ideal choice for researchers aiming to explore the intricate roles of ANP in cardiovascular and renal research. Manufactured under rigorous quality control standards, this kit ensures robust performance and user-friendly protocols, enhancing its value for a wide range of scientific investigations.
| Product Name: | Rat ANP (Atrial Natriuretic Peptide) ELISA Kit |
| Product Code: | AEES00299 |
| Assay Type: | Competitive |
| Format: | 96T |
| Assay Time: | 2.0h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 50?L |
| Specificity: | This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of ANP concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 50?L standard or sample to the wells, immediately add 50?L Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
| 2. | Aspirate and wash the plate for 3 times |
| 3. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 4. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 5. | Add 50?L Stop Solution |
| 6. | Read the plate at 450nm immediately. Calculation of the results |
