The Rat BAX (Bcl-2 Associated X Protein) CLIA Kit is specifically designed for the quantitative detection of BAX levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.BAX is a key protein involved in the regulation of apoptosis, playing a crucial role in cell death and survival pathways. Dysregulation of BAX expression has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it an important target for research and potential therapeutic interventions.
With its reliable performance and user-friendly format, the Rat BAX CLIA Kit is an invaluable tool for studying the role of BAX in various biological processes and disease states. Get precise and insightful results with this advanced assay kit from Assay Genie.
Product Name:
Rat BAX (BCL-2 Associated X Protein) CLIA Kit
SKU:
RTES00059
Target:
Rat BAX (BCL-2 Associated X Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BAX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BAX. You can calculate the concentration of Rat BAX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
103-117
90-105
Average (%)
108
110
97
1:4
Range (%)
91-105
93-104
101-118
Average (%)
98
98
109
1:8
Range (%)
92-109
97-108
85-98
Average (%)
100
103
90
1:16
Range (%)
92-108
93-108
89-100
Average (%)
99
100
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
92-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.25
262.08
808.6
96.93
236.99
888.93
Standard deviation
9.88
27.49
88.62
8.83
17.49
83.91
C V (%)
9.66
10.49
10.96
9.11
7.38
9.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BAX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BAX in samples. No significant cross-reactivity or interference between Rat BAX and analogues was observed.
