Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
The Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit is a highly specialized assay designed to quantitatively detect and measure the levels of bFGF/FGF2 in various biological samples from rats. Basic Fibroblast Growth Factor (bFGF/FGF2) is a crucial growth factor known for its diverse roles in cell proliferation, angiogenesis, wound healing, and tissue regeneration. Its aberrant expression has been associated with several pathological conditions, making accurate measurement of bFGF/FGF2 levels essential for studying its biological functions and potential implications in diseases. This ELISA kit provides exceptional sensitivity and specificity for reliable and reproducible results, allowing researchers to delve deeper into the molecular mechanisms mediated by bFGF/FGF2. By offering robust performance and stringent quality control measures, the Rat bFGF/FGF2 ELISA Kit ensures precise quantification of bFGF/FGF2, thus serving as an indispensable tool for researchers exploring the intricate signaling pathways and therapeutic implications of this growth factor. Trust in the Rat bFGF/FGF2 ELISA Kit to advance your research in cell biology, regenerative medicine, and disease pathogenesis.
Product Name:
Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
SKU:
AEES00321
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
