The Rat C-P (C-peptide) ELISA Kit is specifically designed to empower researchers with the precise and quantitative analysis of C-peptide levels in a variety of biological samples collected from rat models. C-peptide, a crucial peptide derived from proinsulin during insulin biosynthesis, serves as a valuable indicator of endogenous insulin production and pancreatic beta-cell function. By accurately measuring C-peptide concentrations, this ELISA kit facilitates a comprehensive examination of pancreatic beta-cell activity, insulin secretion, and glucose metabolism in rat subjects. Accurate quantification of C-peptide levels is essential for providing insights into pancreatic beta-cell health, insulin secretion patterns, and overall glucose homeostasis. The C-P ELISA kit enables researchers to explore the intricate interplay between C-peptide, insulin, and glucose regulation, offering a deeper understanding of metabolic processes and potential pathological conditions related to diabetes and other metabolic disorders. Manufactured with a focus on precision and reliability, the Rat C-P ELISA Kit ensures exceptional sensitivity, specificity, and reproducibility, guaranteeing robust performance and consistent results. Researchers can rely on this kit to deliver accurate and insightful data, making it an indispensable tool for investigations into pancreatic function and metabolic health in rat models.
Product Name:
Rat C-P (C-peptide) ELISA Kit
SKU:
AEES00520
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
