The Rat C3a (Complement Component 3a) ELISA Kit is a specialized assay designed for the precise quantification of Complement Component 3a levels in a variety of biological samples obtained from rat models. Complement Component 3a is a pivotal protein fragment generated during the activation of the complement system, playing a critical role in inflammation, immunity, and host defense. Accurate measurement of C3a levels is essential for investigating immune responses, inflammatory pathways, and complement system activities. This ELISA kit offers researchers a reliable and high-throughput method to quantify Complement C3a, enabling in-depth exploration of its functions, modulation, and involvement in various pathophysiological processes. By quantitatively assessing C3a, researchers can gain valuable insights into the intricate interplay between the complement system and immune responses, shedding light on its implications for health and disease. The Rat C3a ELISA Kit by Assay Genie is characterized by its exceptional sensitivity and specificity, ensuring the generation of precise and repeatable results. Manufactured under strict quality control standards, this kit guarantees robust and dependable performance, combined with user-friendly protocols, making it an ideal instrument for cutting-edge research endeavors focused on unraveling the complexities of the complement system and its impact on immune regulation.
Product Name:
Rat C3a (Complement Component 3a) ELISA Kit
SKU:
AEES00366
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
