The Rat CASP3 (Caspase 3) ELISA Kit is a high-quality assay designed for the quantitative detection of Caspase 3 levels in rat biological samples. Caspase 3 is a critical enzyme involved in apoptosis (programmed cell death) and plays a significant role in regulating various cellular processes, including cell differentiation, proliferation, and activation of inflammatory pathways. Accurate measurement of Caspase 3 levels can provide insights into apoptotic pathways, cellular response to stress, and the development of various diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. This ELISA kit offers a reliable and precise method to assess Caspase 3 activity, making it an indispensable tool for apoptosis research and drug discovery studies. Built upon stringent quality control standards, Assay Genie's Rat CASP3 ELISA Kit delivers exceptional sensitivity and specificity, ensuring reproducible results with robust performance. With user-friendly protocols and accurate quantification capabilities, this kit is an excellent choice for investigating apoptotic mechanisms and related pathways in research settings.
Product Name:
Rat CASP3 (Caspase 3) ELISA Kit
SKU:
AEES00344
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
