The Rat Collagen Type I (COL1) ELISA Kit is a specialized assay designed to quantitatively detect Collagen Type I levels in rat biological samples.
Collagen Type I is a fundamental component of the extracellular matrix essential for providing structural support and strength to various tissues, including bone, skin, tendon, and ligaments. This ELISA kit serves as a valuable tool for researchers investigating collagen-related processes, tissue remodeling, wound healing, and skeletal biology in rat models. Accurate measurement of Collagen Type I levels is crucial for gaining insights into tissue integrity, regeneration, and the pathophysiology of conditions related to collagen dysregulation. With the Rat COL1 ELISA Kit, researchers can explore the dynamic roles of Collagen Type I in tissue homeostasis and repair, offering a deeper understanding of collagen biology and its implications in various physiological and pathological conditions. Leverage the exceptional sensitivity and specificity of the Rat COL1 ELISA Kit to ensure precise and reproducible results, facilitating in-depth investigations into the intricate functions of Collagen Type I in tissue development, maintenance, and regeneration. Trust in the robust performance of this kit to advance your research in areas concerning extracellular matrix dynamics and tissue physiology.
Product Name:
Rat COL1 (Collagen Type Ⅰ) ELISA Kit
SKU:
AEES00524
Target:
Rat COL1 (Collagen Type Ⅰ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL1. You can calculate the concentration of Rat COL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
93-105
98-107
Average (%)
105
99
102
1:4
Range (%)
88-98
92-103
97-110
Average (%)
93
99
102
1:8
Range (%)
94-103
97-111
88-102
Average (%)
99
103
95
1:16
Range (%)
85-97
85-96
95-106
Average (%)
90
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
97
EDTA plasma (n=5)
95-103
99
Cell culture media (n=5)
88-94
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.63
55.58
244.42
23.19
56.65
218.01
Standard deviation
1.05
2.56
12.27
1.16
2.83
8.72
C V (%)
5.09
4.61
5.02
5.0
5.0
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat COL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL1 in samples. No significant cross-reactivity or interference between Rat COL1 and analogues was observed.
