The Rat COL4 (Collagen Type 4) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of collagen type 4 levels in rat samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing reliable and reproducible results for a variety of research applications.Collagen type 4 is a major component of the basement membrane and plays a crucial role in maintaining tissue integrity and function. Changes in collagen type 4 levels have been associated with various pathological conditions, including fibrosis, diabetes, and kidney disease.
By accurately measuring collagen type 4 levels, researchers can gain valuable insights into disease progression and identify potential therapeutic targets. Overall, the Rat COL4 CLIA Kit is an essential tool for studying collagen type 4 biology and its implications in disease research. Its high sensitivity and specificity make it a reliable choice for researchers looking to explore the role of collagen type 4 in health and disease.
Product Name:
Rat COL4 (Collagen Type 4) CLIA Kit
SKU:
RTES00139
Target:
Rat COL4 (Collagen Type 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL4. You can calculate the concentration of Rat COL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
91-105
91-102
Average (%)
103
99
96
1:4
Range (%)
102-116
90-104
94-106
Average (%)
108
97
101
1:8
Range (%)
93-110
99-115
100-116
Average (%)
100
105
108
1:16
Range (%)
98-112
88-100
95-108
Average (%)
105
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
311.52
547.72
2926.16
304.49
576.52
2906.05
Standard deviation
35.86
41.96
237.6
24.36
65.55
195.0
C V (%)
11.51
7.66
8.12
8.0
11.37
6.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL4 in samples. No significant cross-reactivity or interference between Rat COL4 and analogues was observed.
