The Rat CRP (C-Reactive Protein) CLIA Kit is specifically designed for the quantitative detection of CRP levels in rat serum samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for researchers conducting studies related to inflammation, infection, and cardiovascular diseases.CRP is an important acute phase protein that plays a key role in the body's response to tissue injury and inflammation. Elevated levels of CRP have been associated with various health conditions, including rheumatoid arthritis, lupus, and cardiovascular diseases.
The Rat CRP CLIA Kit provides researchers with a valuable tool for studying the role of CRP in disease progression and developing potential therapeutic interventions.Overall, this kit is a valuable asset for researchers looking to explore the role of CRP in rat models and advance our understanding of inflammatory disorders and related diseases.
Product Name:
Rat CRP (C-Reactive Protein) CLIA Kit
SKU:
RTES00018
Target:
Rat CRP (C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CRP. You can calculate the concentration of Rat CRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
96-110
87-100
Average (%)
105
102
94
1:4
Range (%)
103-116
85-96
92-105
Average (%)
109
91
99
1:8
Range (%)
88-102
102-115
92-108
Average (%)
95
109
100
1:16
Range (%)
94-112
85-98
100-113
Average (%)
102
90
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
100-112
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.45
4.78
0.54
1.46
4.49
Standard deviation
0.05
0.11
0.31
0.05
0.17
0.35
C V (%)
9.43
7.59
6.49
9.26
11.64
7.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CRP in samples. No significant cross-reactivity or interference between Rat CRP and analogues was observed.
