The Rat E-Cad (E-Cadherin) CLIA Kit is specifically designed for the reliable detection of E-Cadherin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a wide range of research studies.E-Cadherin is a critical protein involved in cell adhesion and plays a vital role in maintaining tissue integrity and structure. Dysregulation of E-Cadherin has been implicated in various diseases, including cancer and developmental disorders.
By accurately measuring E-Cadherin levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.This Rat E-Cad CLIA Kit is an indispensable tool for researchers studying cell adhesion, tissue organization, and disease progression in rat models. Its high performance and ease of use make it a valuable asset in both basic research and drug development efforts.
Product Name:
Rat E-Cad (E-Cadherin) CLIA Kit
SKU:
RTES00190
Target:
Rat E-Cad (E-Cadherin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat E-Cad. You can calculate the concentration of Rat E-Cad in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
94-107
85-99
Average (%)
109
101
92
1:4
Range (%)
103-117
92-104
88-103
Average (%)
109
97
94
1:8
Range (%)
101-116
102-115
91-103
Average (%)
109
109
96
1:16
Range (%)
99-116
99-111
96-110
Average (%)
108
104
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.88
263.46
802.73
102.98
258.06
794.5
Standard deviation
10.08
22.87
57.48
10.97
24.34
65.23
C V (%)
9.26
8.68
7.16
10.65
9.43
8.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat E-Cad concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat E-Cad in samples. No significant cross-reactivity or interference between Rat E-Cad and analogues was observed.
