The Rat E-Cadherin (E-Cad) ELISA Kit is a specialized assay utilized for the quantitative measurement of E-Cadherin levels in various rat biological samples. E-Cadherin, a key cell adhesion molecule critical for maintaining tissue integrity and cell-cell adhesion, plays a vital role in cellular processes like cell differentiation, migration, and embryonic development. E-Cadherin functions as a tumor suppressor by regulating cell-cell adhesion and inhibiting cancer cell invasion and metastasis.
Aberrant E-Cadherin expression is often associated with tumor progression, metastasis, and poor clinical outcomes in cancer patients. Quantifying E-Cadherin levels using this ELISA kit provides valuable insights into cell adhesion dynamics, tumor growth, and metastatic potential. The Rat E-Cad ELISA Kit offered by Assay Genie ensures accurate and reliable measurements of E-Cadherin levels in rat samples with exceptional sensitivity and specificity. Stringent quality control measures guarantee robust performance, reproducible results, and user-friendly protocols, making this kit an excellent choice for researchers investigating the role of E-Cadherin in cancer biology, tissue development, and cell behavior in various experimental settings.
Product Name:
Rat E-Cad (E-Cadherin) ELISA Kit
SKU:
AEES00385
Target:
Rat E-Cad (E-Cadherin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat E-Cad. You can calculate the concentration of Rat E-Cad in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
92-108
96-109
Average (%)
104
98
101
1:4
Range (%)
102-116
86-100
96-112
Average (%)
108
93
104
1:8
Range (%)
95-111
88-98
94-108
Average (%)
102
93
99
1:16
Range (%)
95-107
88-101
92-106
Average (%)
101
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.8
0.5
1.4
5.1
Standard deviation
0.03
0.07
0.23
0.03
0.08
0.24
C V (%)
6
5.38
4.79
6.0
5.71
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat E-Cad concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat E-Cad in samples. No significant cross-reactivity or interference between Rat E-Cad and analogues was observed.
