The Rat FE (Ferritin) CLIA Kit is specifically designed for the precise measurement of ferritin levels in rat serum, plasma, and other biological samples. This kit offers unparalleled sensitivity and accuracy, ensuring consistent and dependable results for various research applications.Ferritin is a key protein responsible for storing and releasing iron in the body, playing a critical role in iron metabolism and homeostasis. Abnormal ferritin levels have been associated with various health conditions, including iron deficiency anemia, inflammation, and liver diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.
With its advanced technology and user-friendly protocols, the Rat FE (Ferritin) CLIA Kit from Assay Genie is a valuable tool for researchers looking to study iron metabolism and related disorders in rat models. Order yours today and unlock new insights into the intricate mechanisms of ferritin regulation.
Product Name:
Rat FE (Ferritin) CLIA Kit
SKU:
RTES00337
Target:
Rat FE (Ferritin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FE. You can calculate the concentration of Rat FE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
102-120
103-116
Average (%)
95
110
110
1:4
Range (%)
86-97
88-100
86-97
Average (%)
91
95
92
1:8
Range (%)
88-102
86-100
87-103
Average (%)
95
93
94
1:16
Range (%)
101-115
95-110
95-109
Average (%)
109
103
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.83
4.14
13.7
1.85
4.25
13.27
Standard deviation
0.22
0.42
1.05
0.2
0.42
1.39
C V (%)
12.02
10.14
7.66
10.81
9.88
10.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FE in samples. No significant cross-reactivity or interference between Rat FE and analogues was observed.
