The Rat αFP (Alpha-Fetoprotein) ELISA Kit is meticulously designed to quantitatively detect Alpha-Fetoprotein levels in various rat biological samples. Alpha-Fetoprotein, a well-studied marker protein, is involved in numerous biological processes, particularly during fetal development. Its diagnostic significance in various conditions, including liver diseases and certain cancers, highlights its clinical relevance and importance in research. Accurate measurement of Alpha-Fetoprotein levels is crucial for understanding its role in physiological and pathological states.
This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. Manufactured under stringent quality control standards, this kit provides reliable performance and ease of use, making it a valuable tool for biomarker analysis and biomedical investigations.
Product Name:
Rat alphaFP (Alpha-Fetoprotein) ELISA Kit
SKU:
AEES00341
Target:
Rat αFP (Alpha-Fetoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat αFP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat αFP. You can calculate the concentration of Rat αFP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
97-109
93-106
Average (%)
92
103
99
1:4
Range (%)
100-116
84-95
96-110
Average (%)
107
90
101
1:8
Range (%)
98-116
85-100
96-108
Average (%)
107
92
101
1:16
Range (%)
96-114
87-100
96-110
Average (%)
104
92
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
3.0
9.3
1.0
3.3
8.5
Standard deviation
0.1
0.2
0.4
0.1
0.1
0.4
C V (%)
9.09
6.67
4.3
10.0
3.03
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat αFP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat αFP in samples. No significant cross-reactivity or interference between Rat αFP and analogues was observed.
