The Rat HPX (Hemopexin) CLIA Kit is a powerful tool for the accurate measurement of hemopexin levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Hemopexin is a key protein involved in heme scavenging and transportation, playing a crucial role in protecting cells from oxidative damage caused by free heme. Dysregulation of hemopexin levels has been linked to various diseases, including hemolytic disorders, liver disease, and inflammatory conditions, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
Overall, the Rat HPX (Hemopexin) CLIA Kit provides a reliable and efficient method for investigating hemopexin levels in rat samples, offering valuable insights into the role of this important protein in health and disease.
Product Name:
Rat HPX (Hemopexin) CLIA Kit
SKU:
RTES00286
Target:
Rat HPX (Hemopexin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HPX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HPX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HPX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HPX. You can calculate the concentration of Rat HPX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
84-98
85-96
Average (%)
97
91
91
1:4
Range (%)
85-97
100-112
86-99
Average (%)
92
106
91
1:8
Range (%)
92-109
96-112
96-111
Average (%)
99
103
102
1:16
Range (%)
96-113
94-111
85-96
Average (%)
104
102
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
106
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.63
132.49
470.02
51.68
140.91
486.68
Standard deviation
5.22
9.62
45.69
5.5
16.18
54.36
C V (%)
9.56
7.26
9.72
10.64
11.48
11.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat HPX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HPX in samples. No significant cross-reactivity or interference between Rat HPX and analogues was observed.
