The Rat HSF1 (Heat Shock Factor 1) ELISA Kit is specifically designed for the quantitative detection of HSF1 levels in rat samples such as serum, plasma, and tissue homogenates. This kit is known for its high sensitivity and accuracy, ensuring reliable and consistent results every time, making it perfect for a variety of research applications. HSF1 is a key transcription factor that plays a critical role in the cellular response to stress, including heat shock and other environmental stresses. Dysregulation of HSF1 has been linked to various diseases such as cancer, neurodegenerative disorders, and aging.
Therefore, measuring HSF1 levels accurately is essential for understanding these diseases and developing potential therapeutic interventions. With the Rat HSF1 ELISA Kit, researchers can accurately quantify HSF1 levels in rat samples, allowing for in-depth study and analysis of the role of HSF1 in various physiological and pathological conditions. This kit provides a reliable and efficient tool for researchers looking to advance their understanding of HSF1 and its implications in health and disease.
Product Name:
Rat HSF1 ELISA Kit
SKU:
RTFI00868
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-101%
84-101%
84-100%
EDTA Plasma (n = 5)
88-102%
82-101%
84-98%
Heparin Plasma (n = 5)
91-100%
82-89%
82-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-104
93
EDTA Plasma (n = 5)
87-105
95
Heparin Plasma (n = 5)
88-100
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
