The Rat HSP-60 (Heat Shock Protein 60) CLIA Kit is a reliable and accurate tool for measuring HSP-60 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.HSP-60 is a vital protein involved in cellular stress response and protein folding, playing a crucial role in various physiological processes. Dysregulation of HSP-60 has been implicated in various diseases, including neurodegenerative disorders, autoimmune diseases, and cancer.
Therefore, monitoring HSP-60 levels can provide valuable insights into disease mechanisms and potential therapeutic interventions.The Rat HSP-60 CLIA Kit from Assay Genie is a convenient and efficient solution for researchers looking to study HSP-60 biology in rat models. With its high-quality reagents and easy-to-follow protocol, this kit enables accurate quantification of HSP-60 levels, making it an indispensable tool for advancing scientific discoveries in the field.
Product Name:
Rat HSP-60 (Heat Shock Protein 60) CLIA Kit
SKU:
RTES00278
Target:
Rat HSP-60 (Heat Shock Protein 60)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Hsp-60. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Hsp-60 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Hsp-60, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Hsp-60. You can calculate the concentration of Rat Hsp-60 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
102-118
91-108
Average (%)
103
109
99
1:4
Range (%)
93-109
88-100
93-110
Average (%)
101
94
101
1:8
Range (%)
94-109
89-103
88-99
Average (%)
101
95
94
1:16
Range (%)
98-114
87-99
88-102
Average (%)
104
93
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
92
EDTA plasma (n=5)
99-112
105
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.95
518.97
1385.44
184.65
563.89
1265.86
Standard deviation
17.49
46.71
151.15
15.22
41.05
136.33
C V (%)
8.66
9.0
10.91
8.24
7.28
10.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Hsp-60 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Hsp-60 in samples. No significant cross-reactivity or interference between Rat Hsp-60 and analogues was observed.
