The Rat IL-4 ELISA Kit is a specialized assay meticulously developed to quantitatively measure the levels of Interleukin-4 (IL-4) in a variety of rat biological samples. IL-4 serves as a pivotal cytokine in immune responses, particularly noted for its role in regulating allergy and assisting in the differentiation of T helper cells. Its intricate involvement in modulating the immune system underscores its importance in various immunological processes and conditions. Accurate measurement of IL-4 levels is crucial for gaining insights into immune modulation mechanisms and developing targeted therapeutic interventions. Our IL-4 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit guarantees robust performance and user-friendly operation, making it an ideal choice for researchers aiming to elucidate the functions and impact of IL-4 in immune response regulation.
Product Name:
Rat IL-4 ELISA Kit
SKU:
AEES00296
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
