The Rat IL-23 CLIA Kit is a reliable and accurate tool for detecting IL-23 levels in rat samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides researchers with dependable and reproducible results for a variety of research applications.IL-23 is an important cytokine involved in regulating the immune response and inflammation, with implications in various diseases such as autoimmune disorders, inflammatory conditions, and infectious diseases.
By measuring IL-23 levels, researchers can gain valuable insights into the role of this cytokine in disease pathology and potentially identify new therapeutic targets.Overall, the Rat IL-23 CLIA Kit is a valuable resource for studying the role of IL-23 in rat models and advancing research in immunology and inflammatory diseases.
Product Name:
Rat IL-23 CLIA Kit
SKU:
RTES00347
Target:
Rat IL-23
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-23. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-23. You can calculate the concentration of Rat IL-23 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
85-97
95-111
Average (%)
96
90
102
1:4
Range (%)
96-111
99-116
99-111
Average (%)
103
108
105
1:8
Range (%)
86-98
84-97
93-103
Average (%)
91
91
98
1:16
Range (%)
94-107
94-108
86-99
Average (%)
99
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
103
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.98
43.55
232.32
24.23
47.21
242.47
Standard deviation
2.54
4.3
16.52
3.0
4.15
20.37
C V (%)
9.78
9.87
7.11
12.38
8.79
8.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-23 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-23 in samples. No significant cross-reactivity or interference between Rat IL-23 and analogues was observed.
