The Rat IL2R Beta CLIA Kit is specifically designed for the precise measurement of IL2R Beta levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.IL2R Beta, also known as CD122, is a key receptor involved in the immune response, particularly in regulating T cell activation and proliferation. Aberrant IL2R Beta levels have been linked to various autoimmune diseases, making it a valuable biomarker for studying immune system dysregulation and developing targeted therapies.
With the Rat IL2R Beta CLIA Kit, researchers can confidently analyze IL2R Beta levels in rat samples, aiding in the understanding of immune-related disorders and potential treatment strategies. Partner with AssayGenie for reliable and innovative solutions in immunology research.
Product Name:
Rat IL-2R beta CLIA Kit
SKU:
RTES00622
Target:
Rat IL-2R beta
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2Rβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2Rβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2Rβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-2Rβ. You can calculate the concentration of Rat IL-2Rβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
86-102
99-116
Average (%)
107
93
107
1:4
Range (%)
97-113
87-101
87-98
Average (%)
103
94
93
1:8
Range (%)
101-115
97-110
102-117
Average (%)
108
103
110
1:16
Range (%)
101-120
98-115
93-107
Average (%)
109
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.51
85.8
403.35
46.31
80.27
414.08
Standard deviation
5.09
9.04
39.17
3.74
8.24
46.63
C V (%)
11.18
10.54
9.71
8.08
10.27
11.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-2Rβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-2Rβ in samples. No significant cross-reactivity or interference between Rat IL-2Rβ and analogues was observed.
