The Rat IL-6R CLIA Kit is a powerful tool for measuring levels of interleukin-6 receptor (IL-6R) in rat samples, including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, providing researchers with accurate and reliable results for a variety of study applications.IL-6R is a key component of the interleukin-6 signaling pathway, playing a crucial role in inflammation, immune response, and various disease processes. By measuring IL-6R levels, researchers can gain insights into the pathogenesis of conditions such as arthritis, autoimmune disorders, and cancer, as well as evaluate the efficacy of potential therapeutic interventions.
With its advanced technology and user-friendly design, the Rat IL-6R CLIA Kit is an invaluable asset for studying the role of IL-6R in disease mechanisms and developing novel treatment strategies. Trust AssayGenie for high-quality research tools that deliver accurate and reproducible results.
Product Name:
Rat IL-6R CLIA Kit
SKU:
RTES00520
Target:
Rat IL-6R
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-6R. You can calculate the concentration of Rat IL-6R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
94-106
88-101
Average (%)
94
101
95
1:4
Range (%)
89-101
83-98
93-104
Average (%)
95
90
99
1:8
Range (%)
93-109
88-99
102-115
Average (%)
100
93
107
1:16
Range (%)
102-116
103-118
94-105
Average (%)
108
109
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.69
98.99
426.69
43.12
107.86
446.74
Standard deviation
4.66
8.24
48.56
4.38
8.21
35.47
C V (%)
9.98
8.32
11.38
10.16
7.61
7.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-6R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-6R in samples. No significant cross-reactivity or interference between Rat IL-6R and analogues was observed.
