The Rat Inhibin B ELISA Kit is a reliable and accurate tool designed for the detection of inhibin B levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Inhibin B is a key protein involved in regulating reproductive functions, especially in the control of follicle-stimulating hormone (FSH) levels and the development of healthy sperm in males.
It plays a critical role in various reproductive disorders and infertility, making it a valuable biomarker for studying these conditions and potential treatment strategies.Overall, the Rat Inhibin B ELISA Kit is an essential tool for researchers studying reproductive health and fertility in rats, providing accurate and reliable quantification of inhibin B levels for impactful research outcomes.
Product Name:
Rat INHB (Inhibin B) ELISA Kit
SKU:
RTES00859
Target:
Rat INHB (Inhibin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INHB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INHB. You can calculate the concentration of Rat INHB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
92-108
87-99
Average (%)
99
98
93
1:4
Range (%)
93-108
83-94
81-97
Average (%)
99
88
88
1:8
Range (%)
87-101
80-91
87-97
Average (%)
93
85
92
1:16
Range (%)
88-98
84-96
85-100
Average (%)
93
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.51
139.68
459.54
41.0
129.34
496.13
Standard deviation
2.55
7.15
14.75
2.87
5.57
16.97
C V (%)
5.6
5.12
3.21
7.0
4.31
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat INHB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INHB in samples. No significant cross-reactivity or interference between Rat INHB and analogues was observed.
