The Rat Irisin ELISA Kit is specifically developed for the precise quantitative measurement of irisin levels in various rat biological samples. Irisin is a novel myokine that has gained significant interest due to its potential role in regulating energy metabolism, thermogenesis, and exercise-related physiology. This ELISA kit enables researchers to accurately quantify irisin concentrations, aiding investigations into the physiological effects of this hormone on metabolism and overall health. Accurate measurement of irisin levels using the Rat Irisin ELISA Kit is essential for understanding its involvement in metabolic processes, obesity, exercise physiology, and other related research areas. Researchers can utilize this kit to explore the impact of irisin on energy homeostasis, muscle function, and metabolic disorders, providing valuable insights into potential therapeutic strategies targeting metabolic diseases and related conditions. Assay Genie's Rat Irisin ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly operation, making it an excellent choice for researchers investigating the physiological functions of irisin and its implications in metabolic health.
Product Name:
Rat Irisin ELISA Kit
SKU:
AEES00514
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
