The Rat MBP (Myelin Basic Protein) ELISA Kit is a specialized assay designed for the quantitative detection of Myelin Basic Protein levels in various rat biological samples. Myelin Basic Protein is critically involved in the formation and maintenance of myelin, the protective sheath surrounding nerve cells that enables efficient nerve signal transmission. This protein plays a vital role in the central nervous system, contributing to the insulation and integrity of nerve fibers. By accurately measuring Myelin Basic Protein levels, researchers can gain insights into the integrity of myelin sheaths, demyelinating diseases, and neurological disorders affecting the nervous system. The Rat MBP ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results in studying myelin-related pathologies and neurodegenerative conditions in rat models. Manufactured under strict quality control standards, this kit provides reliable performance and user-friendly protocols, making it a valuable tool for research focused on neural health and disorders associated with myelin dysfunction.
Product Name:
Rat MBP (Myelin Basic Protein) ELISA Kit
SKU:
AEES00488
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
