Rat MHC3/AgB3/H-13 (Major Histocompatibility Complex3) CLIA Kit
The RAT MHC3 AGB3-H-13 Major Histocompatibility Complex3 CLIA Kit is a cutting-edge assay designed for the accurate detection of rat major histocompatibility complex 3 levels in biological samples. This CLIA kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.The major histocompatibility complex 3 (MHC3) is a crucial component of the immune system, playing a key role in antigen presentation and immune response.
Dysregulation of MHC3 expression has been implicated in various diseases, including autoimmune disorders and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and user-friendly format, the RAT MHC3 AGB3-H-13 Major Histocompatibility Complex3 CLIA Kit is an essential tool for researchers and clinicians alike looking to explore the role of MHC3 in health and disease.
Product Name:
Rat MHC3/AgB3/H-13 (Major Histocompatibility Complex3) CLIA Kit
SKU:
RTES00378
Target:
Rat MHC3/AgB3/H-13 (Major Histocompatibility Complex3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MHCâ…¢/AgBâ…¢/H-1â…¢. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MHCâ…¢/AgBâ…¢/H-1â…¢ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MHCâ…¢/AgBâ…¢/H-1â…¢, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MHCâ…¢/AgBâ…¢/H-1â…¢. You can calculate the concentration of Rat MHCâ…¢/AgBâ…¢/H-1â…¢ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
97-112
96-114
Average (%)
109
104
104
1:4
Range (%)
86-101
101-114
101-116
Average (%)
92
108
108
1:8
Range (%)
90-101
87-98
102-119
Average (%)
96
93
110
1:16
Range (%)
85-96
96-109
101-117
Average (%)
90
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.88
11.47
44.05
4.88
10.7
43.39
Standard deviation
0.46
0.93
2.98
0.51
1.05
3.33
C V (%)
9.43
8.11
6.77
10.45
9.81
7.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MHCâ…¢/AgBâ…¢/H-1â…¢ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MHCâ…¢/AgBâ…¢/H-1â…¢ in samples. No significant cross-reactivity or interference between Rat MHCâ…¢/AgBâ…¢/H-1â…¢ and analogues was observed.
