The Rat Motilin (MTL) ELISA Kit is a cutting-edge tool for measuring motilin levels in rat samples, including serum and plasma. With its exceptional sensitivity and specificity, this kit provides accurate and consistent results, making it perfect for various research purposes.Motilin is a key gastrointestinal hormone that regulates smooth muscle contractions in the digestive tract, playing a crucial role in controlling gut motility. Abnormal levels of motilin have been linked to gastrointestinal disorders such as irritable bowel syndrome and gastroparesis, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
With the Rat Motilin (MTL) ELISA Kit, researchers can effectively investigate the role of motilin in gastrointestinal function and disease, advancing our understanding of digestive health and paving the way for innovative therapeutic interventions.
Product Name:
Rat Motilin (MTL) ELISA Kit
Product Code:
RTDL00696
Size:
96 Assays
Target:
MTL
Synonyms:
MLN, MAP, Promotilin, Motilin-associated peptide
Detection Method:
Sandwich
Range:
93.75-6,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
