The Rat Melatonin (MT) ELISA Kit is a specialized assay designed for the quantitative measurement of Melatonin levels in various biological samples. Melatonin, a hormone secreted by the pineal gland in response to darkness, is a key regulator of the circadian rhythm and plays important roles in sleep-wake cycles, antioxidant activity, and various physiological processes. By accurately quantifying Melatonin levels, researchers can gain insights into the circadian system and its impact on various biological functions and health conditions. Utilizing this ELISA kit enables researchers to precisely measure Melatonin concentrations in rat samples, providing valuable information for studies related to sleep disorders, chronobiology, and neuroendocrinology. The assay ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. Manufactured under stringent quality control standards, this ELISA kit offers robust performance and user-friendly protocols, making it an excellent choice for research exploring the role of Melatonin in circadian biology and related fields.
Product Name:
Rat MT (Melatonin) ELISA Kit
SKU:
AEES00303
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
