The Rat NRG-2 (Neuregulin-2) CLIA Kit is specifically designed for the precise measurement of Neuregulin-2 levels in rat serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable tool for various research purposes.Neuregulin-2 is a key signaling protein involved in cell communication and development, particularly in the nervous system.
Its role in regulating neuronal function and synaptic plasticity makes it a crucial biomarker for studying neurological conditions and potential therapeutic interventions.Overall, the Rat NRG-2 CLIA Kit offers researchers a reliable and effective means of analyzing Neuregulin-2 levels, advancing our understanding of neurobiology and potentially leading to the development of novel treatments for neurological disorders.
Product Name:
Rat NRG-2 (Neuregulin 2) CLIA Kit
SKU:
RTES00497
Target:
Rat NRG-2 (Neuregulin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NRG-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NRG-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NRG-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NRG-2. You can calculate the concentration of Rat NRG-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
89-102
92-105
Average (%)
105
96
100
1:4
Range (%)
87-99
97-111
96-111
Average (%)
94
105
104
1:8
Range (%)
90-103
88-102
84-95
Average (%)
97
93
90
1:16
Range (%)
89-103
95-106
88-100
Average (%)
96
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.67
215.16
749.83
87.72
220.55
677.99
Standard deviation
12.06
17.73
80.98
10.28
26.29
44.34
C V (%)
12.87
8.24
10.8
11.72
11.92
6.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NRG-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NRG-2 in samples. No significant cross-reactivity or interference between Rat NRG-2 and analogues was observed.
