Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
The Rat NT-proBNP (N-terminal pro-brain natriuretic peptide) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of NT-proBNP levels in rat samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.NT-proBNP is a biomarker associated with cardiac dysfunction and heart failure, making it a valuable marker for monitoring and diagnosing cardiovascular diseases in animal models.
Measuring NT-proBNP levels can provide insights into cardiac function and help researchers better understand the mechanisms underlying heart failure.With its high sensitivity and specificity, the Rat NT-proBNP CLIA Kit from Assay Genie is a valuable tool for researchers studying cardiac biomarkers and exploring potential therapies for cardiovascular diseases in rat models.
Product Name:
Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit
SKU:
RTES00412
Target:
Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proBNP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NT-proBNP. You can calculate the concentration of Rat NT-proBNP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-120
93-110
89-104
Average (%)
109
100
97
1:4
Range (%)
99-112
97-110
88-101
Average (%)
106
102
94
1:8
Range (%)
92-106
98-115
90-105
Average (%)
100
105
96
1:16
Range (%)
97-109
86-98
87-97
Average (%)
102
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.3
76.12
320.34
45.42
70.81
322.47
Standard deviation
3.68
7.93
33.41
5.67
5.13
33.09
C V (%)
8.91
10.42
10.43
12.48
7.24
10.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NT-proBNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NT-proBNP in samples. No significant cross-reactivity or interference between Rat NT-proBNP and analogues was observed.
