Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
The Rat N-Terminal Pro-Brain Natriuretic Peptide (NT-proBNP) ELISA Kit serves as a valuable tool for the quantitative measurement of NT-proBNP levels in various biological samples obtained from rat models. NT-proBNP is a critical biomarker associated with cardiac function, particularly myocardial strain and heart failure. Monitoring NT-proBNP levels can provide essential insights into cardiovascular health, the progression of heart failure, and the prognosis of cardiovascular diseases. This ELISA kit enables precise and accurate detection of NT-proBNP concentrations, offering researchers a valuable means to investigate and understand cardiovascular pathophysiology and cardiovascular-related disorders.
With the ability to measure NT-proBNP levels, researchers can explore diagnostic and prognostic applications related to heart conditions and cardiovascular health. Manufactured under strict quality control guidelines, the Rat NT-proBNP ELISA Kit delivers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Researchers can rely on this kit to provide robust performance, accurate measurements, and ease of use, making it an ideal choice for cardiovascular research and investigations into heart-related biomarkers.
Product Name:
Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
SKU:
AEES00537
Target:
Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NT-proBNP. You can calculate the concentration of Rat NT-proBNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
88-99
86-100
Average (%)
93
94
94
1:4
Range (%)
94-106
91-102
83-94
Average (%)
98
97
91
1:8
Range (%)
86-98
84-97
91-101
Average (%)
92
90
96
1:16
Range (%)
92-101
92-99
97-108
Average (%)
97
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-105
99
EDTA plasma (n=5)
97-105
102
Cell culture media (n=5)
97-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.27
457.87
1859.66
182.33
410.06
1884.75
Standard deviation
9.58
21.15
91.5
9.32
19.19
88.58
C V (%)
5.2
4.62
4.92
5.11
4.68
4.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NT-proBNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NT-proBNP in samples. No significant cross-reactivity or interference between Rat NT-proBNP and analogues was observed.
