Rat PECAM1 (Platelet/Endothelial Cell Adhesion Molecule) CLIA Kit
The Rat PECAM1 (Platelet Endothelial Cell Adhesion Molecule) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PECAM1 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.PECAM1 is a key molecule involved in cell adhesion and plays a critical role in the interaction between platelets and endothelial cells.
Its expression is implicated in various physiological and pathological processes, including inflammation, thrombosis, and angiogenesis. Therefore, the Rat PECAM1 CLIA Kit serves as a valuable tool for studying these processes and investigating potential therapeutic interventions.
Product Name:
Rat PECAM1 (Platelet/Endothelial Cell Adhesion Molecule) CLIA Kit
SKU:
RTES00457
Target:
Rat PECAM1 (Platelet/Endothelial Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat pecam1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat pecam1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat pecam1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat pecam1. You can calculate the concentration of Rat pecam1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
90-105
103-119
Average (%)
101
98
109
1:4
Range (%)
97-113
89-102
98-109
Average (%)
105
96
103
1:8
Range (%)
92-104
94-108
86-100
Average (%)
98
100
93
1:16
Range (%)
102-119
96-110
93-104
Average (%)
109
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
91
EDTA plasma (n=5)
101-116
108
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.52
74.53
395.83
44.6
74.31
371.05
Standard deviation
4.38
8.85
36.65
5.67
7.2
30.35
C V (%)
10.55
11.87
9.26
12.71
9.69
8.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat pecam1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat pecam1 in samples. No significant cross-reactivity or interference between Rat pecam1 and analogues was observed.
