Rat P1NP (Procollagen Type I N-Terminal Propeptide) CLIA Kit
The Rat PINP (Procollagen Type I N-Terminal Propeptide) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of PINP levels in rat serum, plasma, and tissue samples. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.PINP is a key biomarker for collagen synthesis and bone formation, playing a crucial role in the regulation of bone metabolism.
Abnormal levels of PINP have been associated with various bone disorders such as osteoporosis, osteoarthritis, and skeletal metastases. The Rat PINP CLIA Kit offers researchers a valuable tool for studying these conditions and advancing our understanding of bone health and disease progression.
Product Name:
Rat P1NP (Procollagen Type I N-Terminal Propeptide) CLIA Kit
SKU:
RTES00626
Target:
Rat P1NP (Procollagen Type I N-Terminal Propeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PINP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PINP. You can calculate the concentration of Rat PINP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
86-99
100-113
Average (%)
99
93
107
1:4
Range (%)
86-98
101-117
94-112
Average (%)
91
109
102
1:8
Range (%)
100-115
89-105
92-103
Average (%)
105
96
97
1:16
Range (%)
104-118
92-103
101-114
Average (%)
109
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
96
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
97-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.31
120.63
447.31
52.51
111.94
456.87
Standard deviation
5.14
14.46
46.79
5.85
8.29
42.4
C V (%)
9.64
11.99
10.46
11.14
7.41
9.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PINP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PINP in samples. No significant cross-reactivity or interference between Rat PINP and analogues was observed.
