Rat S100B (S100 Calcium Binding Protein B) CLIA Kit
The Rat S100B (S100 Calcium Binding Protein B) CLIA Kit is a powerful tool for the precise measurement of S100B levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.S100B is a vital protein that plays a key role in the calcium metabolism of cells. It is involved in various cellular processes, including cell growth, differentiation, and survival.
Abnormal levels of S100B have been associated with neurological disorders, brain injuries, and psychiatric conditions, making it a valuable biomarker for investigating these conditions and developing potential therapies.Overall, the Rat S100B CLIA Kit is an essential resource for researchers studying the role of S100B in physiological and pathological processes in rats, offering reliable and insightful data for scientific investigations.
Product Name:
Rat S100B (S100 Calcium Binding Protein B) CLIA Kit
SKU:
RTES00505
Target:
Rat S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat S100B. You can calculate the concentration of Rat S100B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
95-110
88-101
Average (%)
95
101
94
1:4
Range (%)
103-118
92-108
89-100
Average (%)
110
99
94
1:8
Range (%)
99-113
90-105
93-106
Average (%)
106
96
100
1:16
Range (%)
90-106
86-100
95-106
Average (%)
97
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
97
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
98-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.59
127.89
371.75
50.75
127.49
385.21
Standard deviation
6.82
14.0
23.57
6.35
13.09
33.78
C V (%)
12.97
10.95
6.34
12.51
10.27
8.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat S100B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat S100B in samples. No significant cross-reactivity or interference between Rat S100B and analogues was observed.
