Rat S100B (S100 Calcium Binding Protein B) ELISA Kit
The Rat S100B (S100 Calcium Binding Protein B) ELISA Kit is specifically designed for the accurate quantification of S100B levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing reliable and reproducible results for various research applications.S100B is a key protein involved in regulating cell growth, differentiation, and inflammation. Elevated levels of S100B have been implicated in various neurological disorders, making it a valuable biomarker for studying conditions such as brain injury, neurodegenerative diseases, and psychiatric disorders.
By using the Rat S100B ELISA Kit, researchers can gain valuable insights into the role of S100B in these conditions and potentially identify new therapeutic targets for treatment. This kit is easy to use and provides accurate measurements, making it an essential tool for scientists working in the field of neuroscience and neurology.
Product Name:
Rat S100B (S100 Calcium Binding Protein B) ELISA Kit
SKU:
RTES00723
Target:
Rat S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat S100B. You can calculate the concentration of Rat S100B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-105
92-105
Average (%)
93
97
99
1:4
Range (%)
97-109
82-92
91-107
Average (%)
104
87
98
1:8
Range (%)
95-109
81-93
97-113
Average (%)
102
87
105
1:16
Range (%)
99-116
80-93
96-112
Average (%)
106
86
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
95-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.3
463.7
1488.1
188.1
449.2
1497.5
Standard deviation
12.5
25.0
59.5
12.8
23.4
80.9
C V (%)
6.21
5.39
4.0
6.8
5.21
5.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat S100B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat S100B in samples. No significant cross-reactivity or interference between Rat S100B and analogues was observed.
