The Rat TAT (Thrombin-Antithrombin) Complex ELISA Kit is specifically designed for the precise quantification of thrombin-antithrombin complex levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The thrombin-antithrombin complex is a key indicator of the coagulation cascade, reflecting the balance between thrombin production and antithrombin inhibition. Dysregulation of this complex is associated with various clotting disorders, thrombotic events, and inflammatory processes.
By accurately measuring TAT levels, researchers can gain valuable insights into coagulation dynamics and identify potential therapeutic targets for conditions such as disseminated intravascular coagulation and sepsis.Overall, the Rat TAT Complex ELISA Kit is a valuable tool for studying coagulation disorders and exploring novel interventions to modulate thrombin activity and antithrombin function in preclinical rat models.
Product Name:
Rat TAT (Thrombin-Antithrombin Complex) ELISA Kit
SKU:
RTES00799
Target:
Rat TAT (Thrombin-Antithrombin Complex)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TAT. You can calculate the concentration of Rat TAT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-111
87-101
86-100
Average (%)
101
94
92
1:4
Range (%)
85-101
81-96
88-100
Average (%)
92
88
94
1:8
Range (%)
92-106
85-97
90-103
Average (%)
100
91
95
1:16
Range (%)
91-104
86-98
85-96
Average (%)
99
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
3.01
9.71
0.93
3.29
9.5
Standard deviation
0.05
0.17
0.32
0.06
0.16
0.29
C V (%)
4.85
5.65
3.3
6.45
4.86
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TAT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TAT in samples. No significant cross-reactivity or interference between Rat TAT and analogues was observed.
