Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) CLIA Kit
The Rat VCAM-1 (Vascular Cell Adhesion Molecule-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of VCAM-1 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.VCAM-1 is a key molecule involved in cell adhesion and inflammation, playing a critical role in various processes such as immune response and atherosclerosis.
Abnormal levels of VCAM-1 have been linked to several diseases including cardiovascular diseases, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these disorders and potential therapeutic interventions.Overall, the Rat VCAM-1 CLIA Kit is a valuable tool for researchers seeking to accurately measure VCAM-1 levels in rat samples and advance our understanding of the role this molecule plays in health and disease.
Product Name:
Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) CLIA Kit
SKU:
RTES00591
Target:
Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VCAM-1/CD106. You can calculate the concentration of Rat VCAM-1/CD106 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
100-115
94-109
Average (%)
99
108
100
1:4
Range (%)
99-115
87-99
101-114
Average (%)
105
92
106
1:8
Range (%)
97-109
98-114
86-99
Average (%)
104
104
91
1:16
Range (%)
92-106
88-103
91-103
Average (%)
98
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-109
104
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.97
47.07
174.19
20.36
49.77
158.71
Standard deviation
1.6
4.6
18.06
2.02
3.89
13.71
C V (%)
8.01
9.77
10.37
9.92
7.82
8.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Rat VCAM-1/CD106 and analogues was observed.
