Rat VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
The Rat VEGF-A (Vascular Endothelial Cell Growth Factor-A) ELISA Kit is specifically designed for the accurate measurement of VEGF-A levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.VEGF-A is a key protein that plays a critical role in promoting vascular endothelial cell growth and angiogenesis. It is involved in various physiological and pathological processes, including wound healing, tumor growth, and ischemic diseases. Studying VEGF-A levels can provide valuable insights into these biological processes and aid in the development of novel therapeutic interventions.With its reliable performance and ease of use, the Rat VEGF-A ELISA Kit is an essential tool for researchers studying angiogenesis, vascular biology, and related fields. Get accurate and reliable data with this advanced ELISA kit from Assay Genie.
Product Name:
Rat VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
SKU:
RTES01205
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
