Recombinant Human BAI3 Protein (His Tag) (RPES3607)



ELISA Kit Technical Manual

Recombinant Human BAI3 Protein (His Tag)

Background:Human Brain-Specific Angiogenesis Inhibitor 3 (BAI3) is a 177 kDa seven-span transmembrane (TM) protein, which is thought to be a member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids in size. It contains three distinct regions: an N-terminal extracellular domain (ECD) (aa25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type 1 repeat (aa296-508), and one GSP domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one altermate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI3 shares 98% aa identity with mouse BAI3. BAI3 has been reported primarily in the brain, but is also localized to lung, testis, and pancreas. It might be involved in angiogenesis inhibition and suppression of glioblastoma.
Name:Recombinant Human BAI3 Protein (His Tag)
Assay Genie SKU:RPES3607
Synonyms:Brain-Specific Angiogenesis Inhibitor 3; BAI3; KIAA0550
Expression host:Human Cells
Mol Mass:97.5 kDa
AP Mol Mass:9826 kDa
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Thehalose, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:BAI3: May regulate the number of excitatory synapses that are formed on hippocampus neurons. Has no effect on inhibitory synapses. Might be involved in angiogenesis inhibition and suppression of glioblastoma. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
UniProt Protein Details:

Protein type:GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 6q12-q13

Cellular Component: plasma membrane

Molecular Function:GTPase activator activity; protein binding

Biological Process: myoblast fusion; regulation of dendrite morphogenesis

NCBI Summary:This p53-target gene encodes a brain-specific angiogenesis inhibitor, a seven-span transmembrane protein, and is thought to be a member of the secretin receptor family. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. [provided by RefSeq, Jul 2008]
UniProt Code:O60242
NCBI GenInfo Identifier:311033353
NCBI Gene ID:577
NCBI Accession:O60242.2
UniProt Secondary Accession:O60242,O60297, Q2NKN6, Q5VY37, Q9BX54, B7Z1K0,
UniProt Related Accession:O60242
Molecular Weight:172kDa
NCBI Full Name:Adhesion G protein-coupled receptor B3
NCBI Synonym Full Names:adhesion G protein-coupled receptor B3
NCBI Official Symbol:ADGRB3
NCBI Official Synonym Symbols:BAI3
NCBI Protein Information:adhesion G protein-coupled receptor B3
UniProt Protein Name:Adhesion G protein-coupled receptor B3
UniProt Synonym Protein Names:Brain-specific angiogenesis inhibitor 3
Protein Family:Adhesion G protein-coupled receptor
UniProt Gene Name:ADGRB3
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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