Neuronal acetylcholine receptor subunit beta-3(CHRNB3) is a cell membrane protein and belongs to the ligand-gated ion channel (TC 1.A.9) family. CHRNB3 seems to be composed of two different type of subunits: alpha and beta. The CHRNB3 are (hetero) pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of CHRNB3 are encoded by separate genes and have different primary structure. There are several subtypes of neuronal CHRNB3 that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if like muscle alpha-1, they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits.
Product Name:
Human CHRNB3 Recombinant Protein (RPES0318)
Product Code:
RPES0318
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Neuronal acetylcholine receptor subunit beta-3
Accession:
Q05901
Sequence:
Ile25-Leu232
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Neuronal acetylcholine receptor subunit beta-3 is produced by our Mammalian expression system and the target gene encoding Ile25-Leu232 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
25.3 kDa
AP Mol Mass:
38-40 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
