Recombinant Human EphA1 Protein (His Tag) (RPES2956)



ELISA Kit Technical Manual

Recombinant Human EphA1 Protein (His Tag)

Background:Ephrin type-A receptor 1/EphA1 is a glycosylated member of the Eph family of transmembrane receptor tyrosine kinases. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. EphA1 can form pH sensitive cishomodimers on the cell surface. Membrane-bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation. Reverse signaling is propagated through the Ephrin ligand. EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes. EphA1 is upregulated or downregulated in a variety of human carcinomas and is implicated in tumor invasiveness.
Name:Recombinant Human EphA1 Protein (His Tag)
Assay Genie SKU:RPES2956
Synonyms:Ephrin type-A receptor 1; hEpha1; EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH; EPHA1; EPH; EPHT; EPHT1
Expression host:Human Cells
Mol Mass:57.4 kDa
AP Mol Mass:80-85 kDa
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma
UniProt Protein Details:

Protein type:Membrane protein, integral; EC; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function:ATP binding; protein kinase activity; protein kinase binding; transmembrane-ephrin receptor activity

Biological Process: angiogenesis; cell surface receptor linked signal transduction; ephrin receptor signaling pathway; negative regulation of cell migration; negative regulation of protein kinase activity; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of stress fiber formation; protein amino acid autophosphorylation; regulation of GTPase activity; somatic stem cell maintenance

NCBI Summary:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]
UniProt Code:P21709
NCBI GenInfo Identifier:317373566
NCBI Gene ID:2041
NCBI Accession:P21709.4
UniProt Secondary Accession:P21709,Q15405, A1L3V3, B5A966, B5A967,
UniProt Related Accession:P21709
Molecular Weight:51,069 Da
NCBI Full Name:Ephrin type-A receptor 1
NCBI Synonym Full Names:EPH receptor A1
NCBI Official Symbol:EPHA1
NCBI Official Synonym Symbols:EPH; EPHT; EPHT1
NCBI Protein Information:ephrin type-A receptor 1
UniProt Protein Name:Ephrin type-A receptor 1
UniProt Synonym Protein Names:EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH
Protein Family:Ephrin type-A receptor
UniProt Gene Name:EPHA1
UniProt Entry Name:EPHA1_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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