Human beta-Nerve Growth Factor (beta-NGF) was initially isolated in the mouse submandibular gland. The structure of NGF consists of three non-covalently linked subunits, beta and; it has all of the biological activities associated with NGF. It is structurally linked to BDNF and belongs to the cysteine-knot dynasty of growth factors, which have a stable dimeric form. Nerve growth factor (NGF) is a neurotrophic factor that activates the beta-NGF receptor and communicates via it in order to regulate cell development and survival in the sensory and sympathetic nervous systems. Beta-NGF also serves as a B-cell growth and differentiation molecule, promoting B-cell survival. These findings suggest that beta-NGF is a polytropic cytokine, which in addition to its neurotropic effects may play an important role in immune system regulation. Human beta-NGF has 90% sequence homology with mouse protein and cross-species reactivity. Human Beta-NGF/NGFB Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Beta-NGF/NGFB Recombinant Protein (RPES3611)
Product Code:
RPES3611
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB
Accession:
P01138
Sequence:
Ser122-Arg239
Fusion tag:
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 0.04-0.4 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human beta-Nerve Growth Factor is produced by our Mammalian expression system and the target gene encoding Ser122-Arg239 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
13.5 kDa
AP Mol Mass:
15 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 250mM NaCl; pH 7.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
