Human beta-Nerve Growth Factor (beta-NGF) was initially isolated in the mouse submandibular gland. It is made up of three non-covalently linked subunits, beta, which has all of the biological effects ascribed to NGF. BDNF is structurally related to it; NT-3 and -4, as well as other cysteine-knot growth factors, are in the family of stable dimeric structures. Beta-NGF is a neurotrophic factor that activates its receptor beta-NGF and has an important function in the formation and maintenance of the sensory and sympathetic nervous systems. Beta-NGF promotes B cell growth and maturation, as well as enhancing B-cell survival. Our findings suggest that beta-NGF is a pleiotropic cytokine, with potential immunomodulatory effects in addition to its neurotrophic effects. The human beta-NGF protein is similar in sequence to mouse protein and exhibits cross-species reactivity. Human Beta-NGF/NGFB Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Beta-NGF/NGFB Recombinant Protein (RPES3591)
Product Code:
RPES3591
Size:
20 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB
Accession:
P01138
Sequence:
Ser122-Ala241
Fusion tag:
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 0.03-0.3 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Ala241 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
13.4 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 250mM NaCl; pH 7.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
