Recombinant Human PD-L1/B7-H1/CD274 Protein (Fc & Avi Tag) (RPES5173)

SKU:
RPES5173
€376

Description

ELISA Kit Technical Manual

Recombinant Human PD-L1/B7-H1/CD274 Protein (Fc & Avi Tag)

Background:CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death ligand (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Name:Recombinant Human PD-L1/B7-H1/CD274 Protein (Fc & Avi Tag)
Size:20µg
Assay Genie SKU:RPES5173
Synonyms:Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
Species:Human
Expression host:Human Cells
Sequence:Phe19-Thr239
Accession:Q9NZQ7
Mol Mass:54.2 kDa
AP Mol Mass:70-95 kDa
Tag:C-Fc-Avi
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: plasma membrane

Molecular Function:protein binding

Biological Process: cell surface receptor linked signal transduction; immune response; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; negative regulation of interleukin0 production; positive regulation of T cell proliferation; regulation of activated T cell proliferation; response to cytokine stimulus; signal transduction; T cell costimulation

NCBI Summary:This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:Q9NZQ7
NCBI GenInfo Identifier:83287884
NCBI Gene ID:29126
NCBI Accession:Q9NZQ7.1
UniProt Secondary Accession:Q9NZQ7,Q14CJ2, Q2V8D5, Q66RK1, Q6WEX4, Q9NUZ5, B2RBA2 B4DU27,
UniProt Related Accession:Q9NZQ7
Molecular Weight:20,454 Da
NCBI Full Name:Programmed cell death 1 ligand 1
NCBI Synonym Full Names:CD274 molecule
NCBI Official Symbol:CD274
NCBI Official Synonym Symbols:B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information:programmed cell death 1 ligand 1
UniProt Protein Name:Programmed cell death 1 ligand 1
UniProt Synonym Protein Names:B7 homolog 1; B7-H1; CD_antigen: CD274
UniProt Gene Name:CD274
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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