Description
| SKU: | RPCB2377 |
| Size: | 25 μg, 100 μg |
| Tag: | C-His |
| Reactivity: | Human |
| Protein Description: | Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC. |
| Formulation: | Lyophilized from 0.22 μm filtered solution in 25 mM HEPES, 150 mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Gene ID: | 5328 |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P00749-1 |
 | Recombinant Human PLAU/uPA Protein (pro form) was determined by Tris-Bis PAGE under reducing conditions. |
 | The purity of Human PLAU (pro form) is greater than 95% as determined by SEC-HPLC. |
 | Immobilized Human PLAU, His Tag at 0. 5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/mL determined by ELISA. |
