Recombinant Human SerpinG1/C1IN Protein (His Tag) (RPES4741)

SKU:
RPES4741
€142

Description

ELISA Kit Technical Manual

Recombinant Human SerpinG1/C1IN Protein (His Tag)

Background:As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. Serpin G1 is a serine protease inhibitor protein. It is the largest member among the serpin class of proteins. Remarkably, Serpin G1 has a 2-domain structure, unlike most family members. The C-terminal serpin domain is similar to other serpins, and this part of Serpin G1 provides the inhibitory activity. The N-terminal domain is not essential for Serpin G1 to inhibit proteinases and has no similarity to other proteins. The main function of Serpin G1 is the inhibition of the complement system to prevent spontaneous activation. Serpin G1 is an acute phase protein and circulates in blood at levels of around 0.25g/L, whose levels rise 2-fold during inflammation. Although named after its complement inhibitory activity, Serpin G1 also inhibits proteinases of the fibrinolytic, clotting, and kinin pathways. Most notably, Serpin G1 play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. It is also the most important physiological inhibitor of fXIIa, chymotrypsin and plasma kallikrein.
Name:Recombinant Human SerpinG1/C1IN Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4741
Synonyms:Plasma Protease C1 Inhibitor; C1 Inh; C1Inh; C1 Esterase Inhibitor; C1-Inhibiting Factor; Serpin G1; SERPING1; C1IN; C1NH;HAE1;HAE2
Species:Human
Expression host:Human Cells
Sequence:Asn23-Ala500
Accession:P05155
Mol Mass:53.9 kDa
AP Mol Mass:102 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:SERPING1: a protein protease inhibitor (C1-inhibitor) that forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play an important role in regulating complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Mutations of the SERPING1 gene is associated with adult macular degeneration can also cause hereditary angioedema. Binds to E.coli stcE which allows localization of SERPING1 to cell membranes thus protecting the bacteria against complement-mediated lysis. Belongs to the serpin family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: extracellular space; extracellular region

Molecular Function:serine-type endopeptidase inhibitor activity; protein binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; fibrinolysis; platelet degranulation; blood circulation; innate immune response; blood coagulation; complement activation, classical pathway; blood coagulation, intrinsic pathway; aging

Disease: Complement Component 4, Partial Deficiency Of; Angioedema, Hereditary, Type I

NCBI Summary:This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq, Jul 2008]
UniProt Code:P05155
NCBI GenInfo Identifier:124096
NCBI Gene ID:710
NCBI Accession:P05155.2
UniProt Secondary Accession:P05155,Q16304, Q547W3, Q59EI5, Q7Z455, Q96FE0, Q9UC49 A6NMU0, A8KAI9, B2R6L5, B4E1F0, B4E1H2,
UniProt Related Accession:P05155
Molecular Weight:
NCBI Full Name:Plasma protease C1 inhibitor
NCBI Synonym Full Names:serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
NCBI Official Symbol:SERPING1
NCBI Official Synonym Symbols:C1IN; C1NH; HAE1; HAE2; C1INH
NCBI Protein Information:plasma protease C1 inhibitor; serpin G1; C1-inhibiting factor; C1 esterase inhibitor; complement component 1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1
UniProt Protein Name:Plasma protease C1 inhibitor
UniProt Synonym Protein Names:C1 esterase inhibitor; C1-inhibiting factor; Serpin G1
Protein Family:Para-Rep
UniProt Gene Name:SERPING1
UniProt Entry Name:IC1_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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