Thioredoxin-2; also known as TXN2; MTRX and TRX2; is a member of the thioredoxin family. Tryparedoxins (TXN) are thioredoxin-related proteins which; as trypanothione:peroxiredoxin oxidoreductases; constitute the trypanothione-dependent antioxidant defense and may also serve as substrates for ribonucleotide reductase in trypanosomatids. Thioredoxin-2 / TXN2 contains one thioredoxin domain. It is widely expressed in adult (at protein level) and fetal tissues. Human Thioredoxin-2 / TXN2 is a small redox protein important in cellular antioxidant defenses; as well as in the regulation of apoptosis. Thioredoxin-2 / TXN2 has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Thioredoxin-2 / TXN2 could be involved in the resistance to anti-tumor agents. It possesses a dithiol-reducing activity. Thioredoxin-2 / TXN2 plays an important role in protecting the mitochondria against oxidative stress and in sensitizing the cells to ROS-induced apoptosis. Mammalian Thioredoxin-2 / TXN2 is a mitochondrial isoform of highly evolutionary conserved thioredoxins. Thioredoxins are small ubiquitous protein-disulfide oxidoreductases implicated in a large variety of biological functions.
Product Name:
Human Thioredoxin-2/TXN2 Recombinant Protein (RPES2513)
Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation; which can be measured by absorbance at 650 nm. The specific activity is 5-8 A650/min/mg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human TXN2 (Q99757) (Thr 60-Gly 166) was expressed; with a polyhistide tag at the N-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
13.4 kDa
AP Mol Mass:
13 kDa
Formulation:
Lyophilized from sterile PBS; pH 8.3
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
