Description
Recombinant Human Thioredoxin/SASP/TXN Protein
The Recombinant Human Thioredoxin/SASP/TXN Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Thioredoxin/SASP/TXN function and its implications in health and disease.
This product (SKU: RPCB0149) is produced using E. coli and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 12.45 kDa with an observed molecular weight of 15 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Thioredoxin/SASP/TXN Protein |
| SKU: | RPCB0149 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | TRDX, TRX, TRX1, TXN, TRX, TRX1 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Calculated MW: | 12.45 kDa |
| Observed MW: | 15 kDa |
| Gene ID: | 7295 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Thioredoxin/SASP/TXN Protein (RPCB0149), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
| Bio-Activity: |
|
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Trx-1 is the only extracellular occurring thioredoxin, and is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Plasma concentrations of Trx-1 are up to 6 nM. In cells, Trx-1 is localized predominantly in the cytoplasm. Small amounts have been detected in the nucleus and in association with the outside surface of the cells. Biological functions of Trx-1 include growth factor activity, antioxidant properties, a cofactor that provides reducing equivalents, and transcriptional regulation.
 | Recombinant Human Thioredoxin/SASP/TXN Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized recombinant Human TXN Protein at 1μg/mL (100 μL/well) can bind TXN Rabbit mAb with a linear range of 0.976-5.3ng/mL. |
