Description
Recombinant Human Thrombopoietin R/MPL/CD110 Protein
The Recombinant Human Thrombopoietin R/MPL/CD110 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Thrombopoietin R/MPL/CD110 function and its implications in health and disease.
This product (SKU: RPCB2111) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 53.6 kDa with an observed molecular weight of 68-70 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Human Thrombopoietin R/MPL/CD110 Protein |
SKU: | RPCB2111 |
Size: | 100 μg |
Reactivity: | Human |
Synonyms: | TPO-R, CD110, MPL, c-Mpl, MPLV, K12 |
Tag: | C-His |
Expression Host: | HEK293 cells |
Calculated MW: | 53.6 kDa |
Observed MW: | 68-70 kDa |
Gene ID: | 4352 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Thrombopoietin R/MPL/CD110 Protein (RPCB2111), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Bio-Activity: | Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC 50 of 9.1ng/ml determined by ELISA. |
Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.