Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Product Name:
Mouse bNGF/Beta-NGF Recombinant Protein (RPES2556)
Product Code:
RPES2556
Size:
20 µg
Species:
Mouse
Expressed Host:
CHO Stable Cells
Synonyms:
Ngfb
Accession:
NP_001106168.1
Sequence:
Ser 122-Gly 241
Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 1-8ng/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus.
Purity:
> 96 % as determined by SDS-PAGE
Mol Mass:
13.5 kDa
AP Mol Mass:
13.5 kDa
Formulation:
Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
