Description
Recombinant Mouse Pleiotrophin/PTN Protein
The Recombinant Mouse Pleiotrophin/PTN Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Pleiotrophin/PTN biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0758) is produced using HEK293 cells and features a C-6×His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 16.1 kDa with an observed molecular weight of 18-20 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse Pleiotrophin/PTN Protein |
| SKU: | RPCB0758 |
| Size: | 10 μg , 50 μg |
| Reactivity: | Mouse |
| Synonyms: | Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Osteoblast-specific factor 1, OSF-1, |
| Tag: | C-6×His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 16.1 kDa |
| Observed MW: | 18-20 kDa |
| Gene ID: | 19242 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse Pleiotrophin/PTN Protein (RPCB0758), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cerebral cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 μL/well of Recombinant Mouse Pleiotrophin/PTN at 3-8 μg/mL. |
| Reconstitution: | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Pleiotrophin (PTN) is a secreted, strongly heparinbinding, developmentally regulated cytokine. PTN is a highlyconserved protein ， Human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequenceidentity or greater. PTN and midkine share 50% amino acid (aa) sequence identity, share some functions, andconstitute a family. During development, PTN is involved in development of brain, bone, and organsundergoing branching morphogenesis. PTN causes PTPRB dimerization and inactivates its phosphatase activity,which allows increased tyrosine phosphorylation of its substrates. Increased expression of PTN is correlatedwith neuronal development or stresses such as brain ischemia and Parkinson’s disease.
 | Recombinant Mouse Pleiotrophin/PTN Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
